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KMID : 0374019940170040345
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Ewha Medical Journal
1994 Volume.17 No. 4 p.345 ~ p.354
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Effects of Protein and Amino Acid(Glutamine) on the Development
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Abstract
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late two-cell mouse embryos from ICR9Swiss albino) mice were cultured to the blastocyst stage in New MHBS media.
In order to invertigate the effect of protein macromolecule and glutamine on the development of preimplantation embryos, late 2-cell mouse embryos were cultured in media containing bovine serum albumin(BSA), polyvinylpyrolidone(PVP) and glutamine
and
the rate of blastocyst development was observed.
@ES The results were as following:
@EN 1) There were a significant difference in blastocyst development of early and late 2-cell mouse embryos(P<0.05).
2) The development of late-2-cell mouse embryo to blastocyst in the BSA-free but containing glutamine was higher than those of 0.4% BSA, 0.4% PVPO group.
3) The development of late 2 cell mouse embryo in the MHBS containing glutamine was higher than MHBS with BSA but glutamine-free media group(P<0.05).
4) Balstocyst development is higher in media supplemented with glutamine than in media containing no glutamine.
5) These study suggests that the addition of glutamine to the culture media may be a requried for energy substrate for in-vitro development of late 2-cell mouse embryo.
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